DS23 Efficiency and performance of slide stainers during Mohs frozen section processing

Abstract Mohs micrographic surgery (MMS) relies on good-quality, well-stained frozen sections for effective histological analysis. Slides should also be produced as quickly possible. Automation of complementary devices in the laboratory ensures standardization and efficiency improves overall quality performance. Our study evaluated performance Autostainer-XL (Leica) vs. Linistat (Thermo Scientific) slide stainer within a busy MMS unit. The rapidly dries, loads unloads slides automatically allowing Mohs-trained biomedical scientist (BMS) to work parallel cryotomy. stains with haematoxylin eosin 10 min 50 s. In comparison, was much slower required BMS fix manually hotplate, place them industrial methylated spirit, wash water load individual into Linistat, taking 16 45 s complete staining process. Two blinded assessors retrospectively reviewed same set Linistat-stained stained section consistency staining. scoring criteria were based modified UK National External Quality Assessment Service (NEQAS) Cellular Pathology Technique scheme assessment criteria. Each assessor allocated scores between 1 5 mean significantly higher compared (P < 0.05). our unit, up 12 patients undergo daily an average seven per patient at first stage, leading high processing burden laboratory, particularly stage. increased productivity, ability 30 rack, while only able six rack. practice, reduced time taken produce all layer by more than h when stainer. When surveyed, 100% (n = 8/8) preferred comments suggesting that it easy use, quick, precise important device can withstand demands laboratory. offers improved performance, times, process larger number fully customisable automation